Journal: The Journal of Experimental Medicine

Article Title: Elevated levels of placental growth factor represent an adaptive host response in sepsis

doi: 10.1084/jem.20080398

Figure Lengend Snippet: Effect of PlGF deficiency on tissue mRNA/protein levels of inflammatory and hemostatic markers in a mouse model of endotoxemia. PLGF +/+ (WT) or PLGF −/− (KO) male mice were injected i.p. with or without 16 mg/kg LPS. (A) Shown are the results of quantitative real-time PCR analyses (mRNA copy number per 10 6 copies of 18S) of ICAM-1, VCAM-1, E-selectin, P-selectin, COX-2, and PAI-1 in the heart, lung and liver at 24 h. Data are expressed as means + SD of three independent experiments. *, P < 0.05; **, P < 0.01; and ***, P < 0.0001 compared with untreated controls (and where indicated between PlGF-deficient and wild-type mice). (B) Double immunofluorescence staining for activation markers and CD31 in the liver of wild-type mice treated in the absence (WT) or presence of 16 mg/kg LPS (WT/L) and PlGF −/− mice treated with 16 mg/kg LPS (PKO/L) at 24 h. (a) ICAM-1 (green) and CD31 (red). (b) VCAM-1 (green) and CD31 (red). (c) E-selectin (green) and CD31 (red). (d) P-selectin (green) and CD31 (red). (e) COX-2 (red) and CD31 (green). (f) PAI-1 (red) and CD31 (green). Bars: 132 μm; (insets) 42 μm.

Article Snippet: Immunohistochemistry was performed using the following primary antibodies: hamster monoclonal anti–mouse ICAM-1 (Serotec), rat monoclonal anti–mouse VCAM-1 antibody (BD Biosciences), rat monoclonal anti–mouse E-selectin antibody (BD Biosciences), rat monoclonal anti–mouse P-selectin antibody (Millipore), rabbit polyclonal anti–mouse COX-2 antibody (Cayman Chemical), and rabbit polyclonal anti–mouse PAI-1 antibody (Innovative Research Inc.).

Techniques: Injection, Real-time Polymerase Chain Reaction, Double Immunofluorescence Staining, Activation Assay

Journal: Lung cancer (Amsterdam, Netherlands)

Article Title: Combination therapy with anti-programmed cell death 1 antibody plus angiokinase inhibitor exerts synergistic antitumor effect against malignant mesothelioma via tumor microenvironment modulation.

doi: 10.1016/j.lungcan.2023.107219

Figure Lengend Snippet: Fig. 1. MPM cells secreted multiple angiogenic factors. A, Comprehensive analysis of the expression of transcripts of multiple angiogenic factors (VEGFA, PDGFb, FGF2, HGF, ANG1 and IL-8) in human umbilical vein endothelial cell (HUVEC), mesothelial cell (Met-5A) and MPM cell lines (MSTO-211H, H2452, H2052, and H28) by qRT-PCR. Expression of FGF2 transcripts in all MPM cells was higher than in HUVECs. B, Comprehensive analysis of the protein expression of angiogenic factor receptors (VEGFR2, PDGFRβ, FGFR1, c-Met, Tie2 and CXCR1) in HUVEC, human mesothelial cell and MPM cells by immunoblotting. MPM cells expressed higher level of FGFR1 compared with HUVECs. C, Antiproliferative effect of multi-angiokinase inhibitor nintedanib (NTD) in MPM cells. Mesothelial cells and MPM cells were treated with serially diluted NTD for up to 72 h. The relative numbers of viable cells were quantified using CCK-8 assay. Points, mean % viable cells; bars, SEM of at least three independent experiments performed in triplicate.

Article Snippet: Rabbit polyclonal Ab against CXC motif chemokine receptor 1 (CXCR1) (#55450–1- AP) was obtained from Proteintech (Rosemont, IL).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, CCK-8 Assay